Journal of Biology and Medicine: Open Access

Carbonic anhydrase inhibitors (CAi), histone deacetylase inhibitors (HDACi), and Bruton’s tyrosine kinase inhibitors (BTKi) have demonstrated anticancer properties and the ability to target cancer stem cells. To the best of our knowledge, there is currently no existing in vivo or clinical literature investigating the exclusive combination of these three agents as a therapeutic approach for solid tumors. In this case series, we administered a combination of Acetazolamide, Tegretol, and Multi targeted epigenetic therapy (MTET protocol) consisting of quercetin, sodium phenyl butyrate, and Ibrutinib to end-stage patients with cancers of ovarian, colon, glioma, and sarcoma. Following treatment, a substantial reduction in tumor burden was consistently observed in all cases, as evidenced by labs –consisting of tumor markers and liquid biopsies (circulating tumor cells, and circulating DNA)– and imaging (whole body PET/CT). These findings collectively indicate that the combined use of HDACi, CAi, and BTKi as targeted epigenetic modifiers holds significant promise and clinical potential for improving the prognosis of patients with advanced-stage solid tumors across various cancer types.

The potential of microalgae as a source of bioremediation based on wastewater has received increasing interest throughout the world. A freshwater microalga Chlorella vulgaris was investigated for its ability to reduce salinity from six different polychem industrial wastewaters which were diluted in microalgae in three different proportions (namely, 1:6, 1:1 and 2:1). The results reveled that C. vulgaris grew in wastewater of chemical and fertilizer company, obtained value of pH, salinity, DO, salinity and TDS did not change while absorbance value decreased from day 0 to day 30 due to highly acidic nature. For polymer, dye, RO, and petroleum industry wastewater, the absorbance and pH value increase for all concentration. The lowest salinity and TDS for poly, dye, RO and petroleum industry were all at the concentration of 2:1. The result showed that, Chlorella sp. managed to reduce the salinity for wastewater polymer, dye, RO, and petroleum industry, at concentration of 2:1 which were 3.67 %, 4.53 %, 5.4 %, and 4.91 % respectively. The results suggest that the potential of removing nutrient from wastewater by microalgae cultivation as production feedstock.